If your bands are faint, try adding a GC enhancer to your reaction mix or slightly increasing the primer concentration (standard is often around 0.15–0.2 µM ). Pro-Tip: Beyond the Defaults
: Can utilize sequence quality scores to avoid designing primers in unreliable parts of a read. primer3 0.4.0
./src/primer3_core < input_file.txt > output_file.txt If your bands are faint, try adding a